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Journal of Nutrition Vol. 56 No. 3 July 1955, pp. 335-348
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Microbiological, Versus Biological Vitamin B12 Activity in Bovine Rumen Liquor and Feces1 ,2

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M. Moinuddin3 and Orville G. Bentley

Ohio Agricultural Experiment Station, Wooster

Comparative microbiological (L. leichmannii) and biological (rat and chick) vitamin B12 assays of the dried feces, a lyophilized feces extract, and rumen liquor from a rumenfistulated steer were carried out. The vitamin B12 content of rat and chick liver and kidney was determined microbiologically.

A linear relationship was observed between the vitamin B12 activity for L. leichmannii found in the liver and kidney and the levels of crystalline vitamin B12 in the ration. Although the chicks used in this study did not show a growth response to the graded levels of vitamin B12 in the ration, a definite trend for increased vitamin B12 storage in the livers and kidneys was observed as vitamin levels in the ration increased.

On the basis of the criteria used in this study—namely, weight gains and liver vitamin B12 content, it was found that the microbiologically active vitamin B12 or B12-like substances present in the steer feces were fully active for the rat while only 70% of the activity in rumen liquor was utilized. The activity in dried feces and rumen liquor was less effective, however, for chicks, based on liver and kidney storage of vitamin B12.

1 Approved for publication as a journal article No. 61-54 by the Associate Director of the Ohio Agricultural Experiment Station.

2 The studies reported herein were supported in part by the Atomic Energy Commission under contract No. AT (11-1) — 272.

3 Post doctorate fellow.

Manuscript received 17 January 1955.




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