QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hill, R. M. Articles by Holtkamp, D. E. Search for Related Content PubMed PubMed Citation Articles by Hill, R. M. Articles by Holtkamp, D. E.

Storage of Dietary Manganese and Thiamine in the Rat¹

Department of Biochemistry, University of Colorado Medical Center, Denver

1. The livers of 95 rats on different levels of dietary thiamine and manganese have been assayed for thiamine and manganese. The small intestines of 69, and the ceca and colons of 8 pairs of these rats were assayed for manganese.

2. Storage of thiamine in the liver increases with supplementation of manganese and thiamine intakes until dietary levels of 10 mg and 2.0 mg respectively, per rat per day, are reached.

3. Maximum thiamine storage in the liver, 11–16 µg per gram wet weight, is higher than has been reported earlier.

4. There is some evidence that storage of thiamine in the liver occurs more readily in young adult rats (13 weeks) than in older rats (36 weeks).

5. With thiamine intake constant at 0.03 mg per rat per day, manganese supplementation of the diet from a level of 0.03 to 1.0 mg per rat per day increases the storage of thiamine in the liver.

6. Liver storage of manganese increases with increase in dietary intake up to 40 mg of dietary manganese per day. With manganese intake constant at 1 mg per rat per day, thiamine supplementation of the diet from a level of 0.03 to 0.2 mg per rat per day increases (but not statistically significant) the mean storage of manganese in the liver.

7. At low levels of dietary manganese, storage of manganese in the wall of the small intestine is from one-third to two-thirds as much per gram of tissue as in the liver. At dietary intakes above 10 mg per day storage per gram in the small intestine is somewhat greater than in the liver.

8. Storage of manganese in the wall of the cecum and large intestine is as great or greater per gram than it is in the liver.

A preliminary report of part of this work was presented at the April 1950 meeting of the American Chemical Society at Philadelphia, Pa.

² Present address, Research and Development Division, Smith, Kline and French Laboratories, Philadelphia, Pa.

Manuscript received 6 October 1953.