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Enzymes in Protein Depletion

II. Oxidative Enzymes of Heart Ventricle¹

Two Figures

Bureau of Biological Research, Rutgers University, New Brunswick, New Jersey

The cytochrome oxidase, succinic dehydrogenase and DPN-cytochrome c reductase activities of rat heart ventricle have been assayed after the animals were fed a protein-free diet for 49 days. The accompanying sub-acute food restriction, amounting to 41 and 35%, respectively, in two experiments, was controlled with pair-fed animals which received a diet containing 18% of casein. Ad libitum fed animals which were also given the complete diet served as further controls.

Neither protein depletion with its attendant food restriction nor food restriction alone had any significant effect on the unit activities of these three enzymes. The total activities of all of these enzymes were roughly proportional to the total proteins of the heart. The animals fed the basal diet ad libitum had the highest total activity and the largest amount of total protein, whereas the animals fed the protein-free diet had the lowest total activity and the least amount of total protein. The pair-fed controls had an intermediate total enzyme activity and an intermediate amount of protein in the heart.

² Present address: Department of Ophthalmology, USAF School of Aviation Medicine, Randolph Field, San Antonio, Texas.

³ Public Health Service Postdoctoral Research Fellow of the National Institute of Dental Research, 1950–52. Present address: Dental Research Unit, Army Medical Graduate School, Walter Reed Medical Center, Washington, D. C.

Manuscript received 30 October 1953.