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Journal of Nutrition Vol. 52 No. 3 March 1954, pp. 375-381
Copyright © 1954 by American Society for Nutrition
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Availability of Amino Acids to Microorganisms

II. A Rapid Microbial Method of Determining Protein Value1

Two Figures

Millard J. Horn, Amos E. Blum and Madelyn Womack

Bureau of Human Nutrition and Home Economics, Agricultural Research Administration, United States Department of Agriculture, Washington, D. C.

A rapid method for determining the nutritive value of the proteins of processed cottonseeds has been developed by making a microbiological assay, using an enzymatic digest of the meal as the only source of protein in the medium.

The method is based on the observation that the sum of the available amino acids determines the amount of growth of the microorganism. Since no amino acids are used in the basal medium, the method is relatively inexpensive and results can be obtained in a short time.

1 Preliminary report given before the Animal Nutrition Research Council, October 14, 1953, in Washington, D. C.

Manuscript received 30 October 1953.




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