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Journal of Nutrition Vol. 51 No. 1 September 1953, pp. 35-52
Copyright © 1953 by American Society for Nutrition
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Use of Indicators in the Measurement of the Dry Matter Intake of Grazing Animals1

Two Figures

W. A. Hardison2 and J. T. Reid

Cornell University, Ithaca, New York

The adequacy of indicators for measurement of the dry matter intake was examined in a series of trials in which all herbages studied were grazed by and hand-fed to steers. Intake estimates derived from the measurement of indigestibility by the fecal-chromogen method and of fecal outgo by the use of Cr2O3 were similar to the measured intakes when feces representative of those voided during periods of 4 or more days were employed.

As a result of an experiment in which a detailed "grab" fecal sampling plan was employed, it was found that the Cr2O3 content of the feces voided at intervals of the day is extremely variable. This precludes a random sampling of feces for the purpose of estimating intake. However, the nature of the Cr2O3 excretion pattern with respect to time of day indicated that accurate intake estimates could be derived from samples of feces taken at any time provided that the rate of recovery of Cr2O3 is known. Consequently, the adequacy of feces obtained at convenient sampling times was examined. Feces samples procured at 6:00 A.M. and 4:00 P.M. and compounded on an equal wet-weight basis during 7 or more days resulted in reliable estimates of dry matter intake.

Although the general applicability of this sampling procedure to a wide variety of grazing conditions remains to be determined, excellent results have been obtained in subsequent experiments in which conditions different from those of the present experiments were imposed. It is indicated that factors which influence the rate of Cr2O3 recovery from the feces taken in the morning may effect a change in the recovery of Cr2O3 of similar magnitude, though of opposite direction, in the feces taken during the afternoon. The mean rates of recovery of Cr2O3 from the feces taken from grazing steers at 6:00 A.M. and 4:00 P.M. were 71.8 and 129.3%, respectively. Wet-bulking of the feces samples taken at these two times of day resulted in an average recovery of 99.95% of the Cr2O3 ingested by grazing steers.


1 From a portion of a thesis presented by W. A. Hardison to the Graduate School, Cornell University, in partial fulfillment of the requirements for the Ph.D. degree, 1952.

2 Present address: Virginia Experiment Station, Blacksburg.

Manuscript received 8 June 1953.





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