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The Effect of Dietary Amino Acid Levels upon the Oxidation of L- and D-Amino Acids by Kidney Tissue¹

Department of Biological Chemistry, University of Illinois College of Medicine, Chicago

1. Supplementation of a 10 or 15% casein diet with DL-methionine or DL-phenylalanine was found to increase the rate of L-amino acid oxidation in rat kidney.

2. The addition of DL-methionine to a 10% casein diet or increase of the casein content of the diet of 15% resulted in significant increases in D-amino acid oxidase activity. Supplementation of the 15% casein diet with DL-methionine or DL-phenylalanine produced no change in activity.

3. Kidney succinic dehydrogenase activity was not altered by supplementation of the diet with amino acids.

² Fellow of the National Institute of Health, United States Public Health Service. Present address: Biological Research Laboratory, Southern Illinois University, Carbondale, Illinois.

Manuscript received 12 December 1952.