Journal of Nutrition

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Journal of Nutrition Vol. 49 No. 4 April 1953, pp. 579-588
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Further Studies Relating Liver Xanthine Oxidase to Quality of Dietary Protein1

Gerald Litwack, J. N. Williams, Jr., Prema Fatterpaker2, Lillian Chen and C. A. Elvehjem

Department of Biochemistry, University of Wisconsin, Madison

1. Studies with a deficient protein, gliadin, have indicated that the response in liver xanthine oxidase as a basis for measuring protein quality is very sensitive to changes in the state of the protein with respect to its amino acid composition.
2. Calculation of the enzyme response constant has been analyzed and revised to represent a refined value for protein quality.
3. Enzyme studies with casein, whole gliadin, and gliadin supplemented with various amino acids concur very well with similar studies where growth has been used as a criterion.

1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. Supported in part by funds supplied by the National Live Stock and Meat Board, Chicago, Illinois; by a contract between the Office of Naval Research, Department of the Navy, Washington, D. C., and the Regents of the University of Wisconsin; and by a grant from the Research Corporation, New York, N. Y.

2 Fellow of the Williams-Waterman Fund for the Combat of Dietary Diseases.

Manuscript received 10 November 1952.




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