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Vitamin B₁₂ and Folic Acid in Milk Diets¹

Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

The inferior growth of rats fed a mineralized goat's milk diet was found to be the result of a vitamin B₁₂ deficiency. Rat growth equal to that obtained with a cow's milk diet was attained when 0.5 µg of vitamin B₁₂ was added per rat per day to a goat's milk diet, or when 3 µg of vitamin B₁₂ were added per liter of goat's milk.

Folic acid spared a part of the vitamin B₁₂ requirement of the rat fed a mineralized goat's milk diet.

Folic acid and vitamin B₁₂ additions to mineralized goat's milk or cow's milk diets did not influence the rate of hemoglobin formation in the normal or the anemic rat.

A dry, scaly dermatosis was observed upon the paws of weanling rats fed a goat's milk or a cow's milk diet.

² Present address: International Minerals and Chemical Corporation, Central Research Laboratory, Skokie, Illinois.

Manuscript received 8 August 1952.