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Evaluation of Nitrogen Source Materials by an Intraperitoneal Rat Test¹

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Sterling-Winthrop Research Institute, Rensselaer, New York

A method is described whereby nitrogen source materials intended for intravenous alimentation may be evaluated by intraperitoneal administration to adult rats, which have been protein depleted for one week. In its simplest form, the preparations are administered at two levels for 4 days, excreta collections being made during the last three days. The excreta may be pooled for analysis.

Evaluation of a 6% gelatin solution by both parenteral and oral test indicated it to have about the same biological value, 0.15 to 0.19, thus leaving open the question as to the nutritive value of the contained peptide-bound amino acids.

Five commercial preparations of known clinical usefulness when parenterally administered were observed to have biological values in the range of 0.44 to 0.63.

The data show conclusively that evaluations based upon multiple levels of test may be materially different from those based upon single level tests in conjunction with the nitrogen excretion at zero nitrogen intake. The use of multiple levels thus appears to be mandatory for valid assays.

Revision accepted 16 July 1952.