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New York State College of Home Economics and the School of Nutrition, Cornell University, Ithaca
Six young women 20 through 31 years of age were maintained for 12 weeks on a diet which was constant except for the addition of a serving of spinach a day in the last 8 weeks. The nitrogen content of the urine, feces, menses and duplicates of the food was determined.
The mean intake of protein on the basal diet was 67.0 gm, on the diet containing spinach 71.8 gm, and on both diets for all 12 weeks 70.2 gm. The mean amount of nitrogen retained by the 6 subjects during 12 weeks was 0.64 gm per day, or 0.61 gm if the loss of nitrogen in the menses was deducted. The amount retained varied widely from week to week for a given subject but the means for 12 weeks were similar for all subjects. A satisfactory amount of nitrogen was retained despite the omission of all animal protein from the breakfast.
Approximately 91% (S.E., 4.6%) of the nitrogen of spinach was absorbed.
Manuscript received 15 February 1952.