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Division of Animal Nutrition, University of Illinois, Urbana
Twelve male Holstein calves were employed in a study of the riboflavin requirement during the first 10 weeks of life. Riboflavin levels were tested over a range of 0.30 to 1.80 µg per gram of dry matter in a "synthetic milk" diet containing sulfathalidine. When fed at the rate of 4.5 l of diet per 100 lb. body weight per day, no increase in growth rate was obtained at any level beyond 1.05 µg per gram of dry matter. Excellent growth was observed in a calf fed the diet ad libitum at this level of riboflavin. The results indicate that the riboflavin requirement of the Holstein calf up to 10 weeks of age is approximately 1.0 µg per gram of dry matter consumed.