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New York State College of Home Economics and the School of Nutrition, Cornell University, Ithaca
Six girls between 13 and 14 years of age lived on a controlled diet for 9 weeks. The first two weeks comprised a preliminary period. During the first experimental period of three weeks the mean daily intake of nitrogen was 12.20 gm, corresponding to 76.2 gm of protein. The mean daily amount of nitrogen retained from the diet by the 6 subjects was 1.42 gm, or 0.97 gm per m2, or 29.9 mg per kilogram. During the second experimental period of 4 weeks the mean daily intake was raised to 13.97 gm, corresponding to 87.3 gm of protein. On this diet the mean daily amount of nitrogen retained was 2.17 gm, or 1.45 gm per m2, or 43.7 mg per kilogram.
An estimate was made of the amount of nitrogen which needs to be retained for the requirements of growth at this age. The absence of information regarding the amount of nitrogen needed to replace the losses in hair and nasal excretions, and other losses not usually measured in balance experiments, made an assessment of the exact amount of nitrogen required by the subjects impossible. The mean intake of 76.2 gm of protein per day was probably adequate for these subjects. The intake of 87.3 gm of protein was undoubtedly more than adequate.
Manuscript received 30 April 1951.