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Studies of Vitamin B₁₂, Choline and Related Factors in the Diets of Chicks¹

Lederle Laboratories Division, American Cyanamid Company, Pearl River, New York

1. Choline deficiency as produced by a purified diet was studied in chicks which were also deficient in vitamin B₁₂. The requirement of choline for maximum growth was found to be greater in the absence of vitamin B₁₂ than in its presence. However, the amount of choline required for the prevention of perosis was not decreased by supplying vitamin B₁₂.

2. Betaine did not have a marked effect when added to diets containing an insufficient level of choline.

3. A methionine-deficient diet was fed to vitamin B₁₂-deficient chicks and no evidence of a sparing effect of vitamin B₁₂ on the methionine requirement was noted under the experimental conditions encountered.

4. Choline deficiency was not encountered on a diet of natural foods containing a high level of soybean meal.

Manuscript received 2 December 1950.