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Journal of Nutrition Vol. 37 No. 4 April 1949, pp. 443-456
Copyright © 1949 by American Society for Nutrition
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The Influence of Coprophagy on the Biotin and Folic Acid Requirements of the Rat1

V. H. Barki, P. H. Derse, R. A. Collins, E. B. Hart and C. A. Elvehjem

Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

1. Rats fed purified diets and kept in coprophagypreventing tube cages grew significantly better when folic acid and biotin were added to the diet than when these vitamins were omitted. Inositol, p-aminobenzoic acid, and niacin did not affect the growth rate of these rats. Biotin and folic acid produced only slight and irregular improvement in the growth of rats kept in ordinary screen-bottom cages.
2. The separate effects of coprophagy-prevention and the addition of sulfasuxidine were reflected also in the biotin and folic acid content of the liver, but not in the nicotinic acid content.

1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. This work was supported in part by funds granted by the National Dairy Council, Chicago, on behalf of the American Dairy Association, and in part by funds provided by the Evaporated Milk Association, Chicago. We are indebted to Merck and Company, Rahway, New Jersey, for supplies of the synthetic B vitamins and {alpha}-tocopherol, and to the Winthrop Chemical Company, Inc., New York, N. Y., for crystalline vitamin D2.

Some of the work involved in this research was carried out in cooperation with Dr. H. Nath, a Government of India Research Fellow.

Manuscript received 20 October 1948.




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