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Journal of Nutrition Vol. 37 No. 3 March 1949, pp. 353-360
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Some Properties of an Unidentified Growth Factor Present in Fish Products1

J. M. Pensack2, R. M. Betke and D. C. Kennard

Departments of Animal Science and Poultry Science, Ohio Agricultural Experiment Station, Wooster

The essential chick growth factor present in sardine fish meal and condensed fish solubles was soluble in 75% acetone and in 80% ethanol. It was insoluble in n-butanol. The factor was adsorbed by Darco G-60 at pH 3.0 from a water solution of an alcoholic extract of sardine fish meal or condensed fish solubles, but not adsorbed from an 80% ethanol solution of the same extract. Elution of the factor from Darco G-60 can be accomplished with 10% ammonium hydroxide in 95% ethanol with about 20% loss of activity. The factor was colorless in solution.

1 Published with the approval of the Director of the Ohio Agricultural Experiment Station. Supported in part by a grant from the Commerical Solvents Corporation, New York, N. Y.

2 Present address: Commerical Solvents Corporation, Terre Haute, Indiana.

Manuscript received 7 October 1948.




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