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Growth Efficiency of Essential Amino Acids Alone and in Combination with Casein^1,2,

One Figure

Department of Agricultural Biochemistry, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick

1. When the crystalline essential amino acids (supplied at the minimum levels suggested by Rose, '37) were the sole source of nitrogen, subnormal growth was obtained in the white rat.

2. Supplementation of 2, 4, 6 and 8% levels of casein with crystalline essential amino acids to meet the minimum suggested levels resulted in a progressive increase in growth rate, the highest rate, 3.9 gm per day, being noted at the 8% level of casein.

3. An increase in the quantity of essential nitrogen (supplied by the crystalline essential amino acids as the sole source of nitrogen) so that it was equal to the essential and nonessential nitrogen supplied by the 8% casein diet supplemented with crystalline acids, failed to cause an increase in growth rate significantly greater than that observed when the crystalline acids at their minimum levels were used.

² Presented before the Division of Biological Chemistry, American Chemical Society, New York, N. Y., September, 1947.

Manuscript received 28 June 1948.