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Journal of Nutrition Vol. 35 No. 4 April 1948, pp. 419-424
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False High Values for Ascorbic Acid in Guava Juice

A Note on the Use of the Colorimetric Method with 2,4-Dinitrophenylhydrazine1

Eva Hartzler2

Nutrition Department, Hawaii Agricultural Experiment Station, Honolulu, Hawaii

Determinations of the ascorbic acid content of stored guava juice by the colorimetric method gave values which: (1) were considerably higher than those obtained by dye-titration and (2) indicated little or no loss of ascorbic acid during storage of the juice. Samples of guava juice given to human subjects who were receiving an ascorbic acid-low diet resulted in urinary excretion values which corresponded to the assay values obtained by dye-titration.

The colorimetric method, therefore, gave false high values for the ascorbic acid content of the stored guava juice.

1 Published by permission of the Director of the Hawaii Agricultural Experiment Station as technical paper no. 161.

2 Present address, Department of Agricultural and Biological Chemistry, The Pennsylvania State College, State College, Pennsylvania.

Manuscript received 22 December 1947.




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