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Relation of Carbohydrate to Intestinal Synthesis of Biotin and Hatchability in Mature Fowl^1,2,

Five Figures

Departments of Poultry Husbandry and Biochemistry, University of Wisconsin, Madison

Evidence is presented to show that dextrin favors the intestinal synthesis of biotin in the mature fowl and sucrose does not promote the synthesis of this vitamin. The results obtained with lactose and dried whey indicate that lactose does not stimulate the synthesis of biotin in the intestinal tract of the laying pullet.

The laying hen is a desirable animal for studying intestinal synthesis. A determination of the vitamin content of the egg offers a method of evaluating the extent of synthesis and subsequent absorption of vitamins from the intestinal tract which is difficult in other animals.

There is a failure of embryonic development when the egg yolk contains as little as 50 mµg of biotin per gram on a fresh weight basis. Eggs which contain 150 mµg of biotin per gram will support normal embryonic development.

² Supported in part by a grant from the Western Condensing Co., San Francisco, Calif.

³ General Education Board Fellow.

Manuscript received 18 August 1947.