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A Bioassay for Proteins and Protein Digests

Three Figures

Wyeth Institute of Applied Biochemistry, Philadelphia, Pennsylvania

An assay for the evaluation of the nutritional quality of proteins and protein digests has been described. The assay is based on the determination under standardized conditions of the amount of fed protein or protein digest nitrogen necessary daily to maintain a constant weight in adult rats that have been partially depleted of body protein. Rats are fed a protein-free diet for 1 week; various groups are then fed varying levels of each test sample for the second week. The daily allowance of ration is proportional to the average surface area of the rats. By plotting body weight change during the second week against daily nitrogen intake, it is possible to interpolate the amount of nitrogen necessary to maintain constant weight in the partially depleted animal. Each test substance is evaluated by comparison with a casein standard.

² Present address: Wyeth Inc., Nutritional Division, Mason, Michigan.

Manuscript received 8 May 1947.