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On the Growth-Promoting Factor for Rats Present in Summer Butter

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Netherlands Institute of Nutrition and Biochemical Laboratory, University of Amsterdam, Holland

Earlier experiments, in which we found a better growth in young albino rats on summer butterfat than on vegetable oils, were confirmed. The growth-promoting action was mainly present in the saponifiable fraction, that is, in the fatty acids. On fractionating the methyl esters of the acids of butterfat by distillation in vacuo, the activity was found in the C₁₈-fraction. On fractionating first with lead acetate and then with mercuric acetate we obtained a fraction chiefly containing vaccenic acid. This fraction possessed distinct growth-promoting qualities. It seems highly probable therefore that vaccenic acid is a growth-promoting factor present in summer butter.