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Bodily Storage of Vitamin A in Relation to Diet and Age, Studied by the Assay Method of Single Feedings¹

Department of Chemistry, Columbia University, New York

In experiments with rats, a diet furnishing 3 I.U. of vitamin A per gram of air-dry food (0.8 I.U. per Calorie) has supported growth and life histories that are within the normal range but has resulted in the storage of only negligible reserves in the liver.

Parallel animals on diets containing, respectively, twice and four times as much vitamin A, acquired larger stores (and higher with the higher intake level) both per gram of liver and in total amount.

At the highest of these levels the body store increased steadily throughout the age range of the experiment (28 to 300 days). At the intermediate level there was a slackening of the storage trend at about 90 days, as if requirement were greatest at about that age.

In no case were differences in storage as great as if all the theoretically potential surplus had been stored, the actual efficiency of storage being of the order of 10 to 30% of the theoretically available surplus ingested in the food.

Manuscript received 27 June 1945.