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Journal of Nutrition Vol. 30 No. 2 August 1945, pp. 67-79
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Physiological Availability of the Vitamins

I. The Human Bioassay Technic1

One Figure

Daniel Melnick, Melvin Hochberg and Bernard L. Oser

Food Research Laboratories, Inc., Long Island City, New York

A biological assay technic has been described for the simultaneous determination of the physiological availability of the water-soluble vitamins, ascorbic acid, thiamine, riboflavin and nicotinamide. The test is based upon the observation that the urinary excretion of the water-soluble vitamins, as such or as their derivatives, parallels the quantity consumed provided that normal test subjects are employed and that the subjects at the time of the tests are subsisting on an adequate diet. A simplified feeding routine is described which extends the applicability of the assay to quantitative studies of problems in human nutrition, particularly those dealing with the factors which contribute to conditioned malnutrition. The dose-response relationships for each of the test vitamins have been found to be linear and the reproducibility of the data superior to that encountered in most animal assays.

1 This paper is one in a series of reports dealing with the physiological availability of the vitamins. It was presented in part before the Division of Biological Chemistry at the 108th meeting of the American Chemical Society, New York, N. Y. The expenses of this study were defrayed by a grant from Lever Brothers Company, Cambridge, Massachusetts.

Manuscript received 11 April 1945.




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