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The Biological Determination of Vitamin E^1,2,

Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

1. A new low-fat diet was shown to be satisfactory for vitamin E assays.

2. A new criterion, viz., the increase in weight during gestation, was shown to be valid as a basis for the determination of vitamin E.

3. The new method was found to be more sensitive and more economical than others heretofore reported.

4. Synthetic -, ß- and -tocopherols and -tocopheryl acetate were found to have relative biological activities of 100, 25, 19 and 100, respectively.

5. For an equal response the requirement for vitamin E on the low-fat diet was less than half of that on a high-fat diet.

² The authors are indebted to the Lever Brothers Company and to the Wisconsin Alumni Research Foundation for grants in support of this work.

Manuscript received 9 November 1942.

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