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A Modification of the Line Test Applicable to Chicken Vitamin D Assay

Four Text Figures and Two Plates (Six Figures)

Biological Laboratories, Winthrop Chemical Company, Inc., Rensselaer, New York

1. An adaptation of the U.S.P. line test to chick bones is described. The method permits a rapid and accurate determination of the index of calcification and an excellent correlation between the degree of calcification and dietary supplements of vitamin D.

2. A typical chicken vitamin D assay is given including data on both the index of calcification and the per cent bone ash.

3. It is shown that both criteria would lead to substantially the same result for the potency of the various preparations of vitamin D tested.

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