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Journal of Nutrition Vol. 17 No. 3 March 1939, pp. 269-280
Copyright © 1939 by American Society for Nutrition
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The Distribution of Vitamin B1 (Thiamin) in Meat and Meat Products1

Olaf Mickelsen, Harry A. Waisman and C. A. Elvehjem

Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

1. A biological method of assaying various materials for their vitamin B1 content has been described. This is based on a comparison of the growth of rats on a basal ration containing the material to be assayed with the growth of rats on the basal ration with added amounts of crystalline vitamin B1.
2. A simple method of drying moist materials, which minimizes destruction of vitamin B1, has also been described.
3. By our method of assay a large number of meats and meat products have been tested for their vitamin B1 content. A study has also been made on the stability of the vitamin under various types of household cooking. Here it was found that there was slight destruction of the vitamin during frying, but with roasting, broiling or stewing, the destruction of the vitamin approached 50%.
4. A study of the influence of fats on the growth of the rats on our basal ration has been made. Our work showed that levels of fat up to 20% had no influence on the validity of our assays.


1 Published with the approval of the director of the Wisconsin Agricultural Experiment Station.

Supported in part by a grant from the National Live Stock and Meat Board made through the National Research Council.

Manuscript received 14 November 1938.





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