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Toxicity of Aspergillus Sydowi and Its Correction¹

Five Figures

Departments of Biochemistry and Agricultural Bacteriology, College of Agriculture, University of Wisconsin, Madison

The nutritive failure of rats fed on a ration in which all protein and B vitamins were supplied by the mycelium of Aspergillus sydowi is not the result of an amino acid deficiency. Addition of liver to the basal was the most effective means of rendering the ration suitable for growth; casein was less effective, and lactalbumin, egg white, edestin and mixtures of certain amino acids were only slightly effective. Fractionation of a water extract of liver indicated that the potency resided in a high molecular weight substance. The factor was destroyed by autoclaving or by acid hydrolysis. Vitamin B supplements were ineffective.

The ration could also be made suitable for growth by the addition of Lloyd's reagent. Other adsorbents were not effective. These results indicated that there was a toxic substance in the mold. Further proof of this theory was that it was possible to obtain growth on the mold ration by adsorbing a water extract of the mycelium with Lloyd's reagent.

Manuscript received 1 July 1938.