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Studies on the Vitamin B₁ Requirements of Growing Chicks^1,2,

Three Figures

Department of Agricultural Chemistry, University of Wisconsin, Madison

1. The vitamin B₁ requirement of the chick as measured by the prophylactic technic is remarkably constant.

2. When ration 242A is supplemented with 20 to 25 international units of vitamin B₁ supplied by the international standard acid clay adsorbate the chicks are protected from polyneuritis.

3. Assays on samples of crystalline synthetic vitamin B₁ indicate that chicks are protected from polyneuritis when they are fed 60 micrograms of vitamin B₁ hydrochloride (sample 1) per 100 gm. of ration 242A. A second sample (sample 2) possessed lower antineuritic potency since 80 micrograms of crystalline vitamin B₁ hydrochloride per 100 gm. of ration 242A were required to protect the chicks from polyneuritis.

² Presented at the ninety-fourth meeting of the American Chemical society at Rochester, September 1937.

³ Wilson and Company Fellow.

Manuscript received 18 October 1937.