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Further Studies on the Growth Promoting Factor Associated with Summer Milk¹

One Figure

Department of Agricultural Chemistry, University of Wisconsin, Madison

1. A series of supplements was fed to rats receiving mineralized winter milk ad libitum. Growth was stimulated by daily supplements of 3 gm. fresh grass, 3 cc. grass juice, 0.6 gm. dried oat grass, 0.2 gm. rice bran, or 0.2 gm. liver extract.

2. The known essential food factors, such as vitamins A, D, C, B, B₂, B₄ and B₆, as well as flavin, choline, ‘goat milk factor,’ and ‘alcohol-ether precipitate factor,’ have been eliminated from consideration as being the active principles, by the inferior growth responses produced by daily doses of 2 drops of cod liver oil, 1 cc. orange juice, 0.5 gm. brewers' yeast, 0.25 gm. dried brain, and 1.0 gm. defatted wheat germ.

3. Increased growth of rats on mineralized winter milk receiving a grass, or grass juice, supplement over that of controls receiving no supplement is accompanied by an increase in milk consumption.

Supported in part by a grant from the Research Fund of the University.

Manuscript received 28 January 1937.