Journal of Nutrition EB Program 2010 Early Registration

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

J. Nutr. First published June 17, 2009; doi:10.3945/jn.109.105205
 Journal of Nutrition, doi:10.3945/jn.109.105205
Vol. 139, No. 8, 1525-1533, August 2009
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Supplemental Material
Right arrow All Versions of this Article:
139/8/1525    most recent
jn.109.105205v1
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Schepens, M. A. A.
Right arrow Articles by Bovee-Oudenhoven, I. M. J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schepens, M. A. A.
Right arrow Articles by Bovee-Oudenhoven, I. M. J.
© 2009 American Society for Nutrition

Nutrition and Disease

Supplemental Calcium Attenuates the Colitis-Related Increase in Diarrhea, Intestinal Permeability, and Extracellular Matrix Breakdown in HLA-B27 Transgenic Rats1–3,

Marloes A. A. Schepens4,5, Arjan J. Schonewille4,5, Carolien Vink4,5, Evert M. van Schothorst4,6, Evelien Kramer4,6, Thijs Hendriks7, Robert-Jan Brummer4, Jaap Keijer6, Roelof van der Meer4,5 and Ingeborg M. J. Bovee-Oudenhoven4,5,*

4 TI Food and Nutrition, 6700 AN Wageningen, The Netherlands; 5 NIZO Food Research, 6710 BA Ede, The Netherlands; 6 RIKILT-Institute of Food Safety, Wageningen University and Research Centre, 6700 AE Wageningen, The Netherlands; and 7 Department of Surgery, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands

We have shown in several controlled rat and human infection studies that dietary calcium improves intestinal resistance and strengthens the mucosal barrier. Reinforcement of gut barrier function may alleviate inflammatory bowel disease (IBD). Therefore, we investigated the effect of supplemental calcium on spontaneous colitis development in an experimental rat model of IBD. HLA-B27 transgenic rats were fed a purified high-fat diet containing either a low or high calcium concentration (30 and 120 mmol CaHPO4/kg diet, respectively) for almost 7 wk. Inert chromium EDTA (CrEDTA) was added to the diets to quantify intestinal permeability by measuring urinary CrEDTA excretion. Relative fecal wet weight was determined to quantify diarrhea. Colonic inflammation was determined histologically and by measuring mucosal interleukin (IL)-1β. In addition, colonic mucosal gene expression of individual rats was analyzed using whole-genome microarrays. The calcium diet significantly inhibited the increase in intestinal permeability and diarrhea with time in HLA-B27 rats developing colitis compared with the control transgenic rats. Mucosal IL-1β levels were lower in calcium-fed rats and histological colitis scores tended to be lower (P = 0.08). Supplemental calcium prevented the colitis-induced increase in the expression of extracellular matrix remodeling genes (e.g. matrix metalloproteinases, procollagens, and fibronectin), which was confirmed by quantitative real-time PCR and gelatin zymography. In conclusion, dietary calcium ameliorates several important aspects of colitis severity in HLA-B27 transgenic rats. Reduction of mucosal irritation by luminal components might be part of the mechanism. These results show promise for supplemental calcium as effective adjunct therapy for IBD.

* To whom correspondence should be addressed. E-mail: ingeborg.bovee{at}nizo.nl.

Manuscript received 4 February 2009. Initial review completed 16 March 2009. Revision accepted 26 May 2009.

Published online 17 June 2006.






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 2009 by American Society for Nutrition