QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Online Supplemental Material All Versions of this Article: 139/7/1410    most recent jn.108.102269v1 Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Damsgaard, C. T. Articles by Frøkiær, H. PubMed PubMed Citation Articles by Damsgaard, C. T. Articles by Frøkiær, H.

---

Nutritional Immunology

Reduced ex Vivo Interleukin-6 Production by Dietary Fish Oil Is Not Modified by Linoleic Acid Intake in Healthy Men^1–3,

⁴ Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen, Frederiksberg DK-1958, Denmark; ⁵ Institute of Human Nutrition, School of Medicine, University of Southampton, Southampton SO16 6YD, UK; and ⁶ Center of Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby DK-2800, Denmark

Fish oil (FO) is considered antiinflammatory, but evidence regarding its effect on human cytokine production is conflicting. High linoleic acid (LA) intake may impair any effects of FO. The aim of this study was to investigate how FO combined with high or low LA intake affected ex vivo cytokine production from cultures of whole blood, peripheral blood mononuclear cells (PBMC), and monocytes in healthy men. The study was a double-blinded, controlled, 2 x 2 factorial 8-wk intervention. Sixty-four healthy men were randomized to 5 mL/d FO or olive oil (OO) provided in capsules and to spreads and oils with high or low LA content, resulting in LA intakes of 7 ± 2% and 4 ± 1% energy, respectively. We measured eicosapentaenoic acid (EPA) in PBMC and stimulated cytokine production in whole blood and PBMC 24-h cultures before and immediately after intervention and after an 8-wk wash-out period, and in monocyte cultures immediately after intervention. PBMC-EPA was markedly increased by FO (P < 0.001). LA intake did not modify the incorporation of FO and tended to have only a slight effect on PBMC-EPA by itself (P = 0.06). Lipopolysaccharide (LPS)-stimulated whole-blood interleukin (IL)-6 production immediately after intervention was lower with FO than OO (P = 0.02) but did not correlate with PBMC-EPA in the FO groups (r = –0.12; P = 0.53; n = 31). The LA intake did not modify IL-6 production or the effect of FO. Neither FO nor LA intake affected the production of tumor necrosis factor-, IL-10, or interferon- in any of the cultures. In conclusion, FO intake reduced IL-6 production from LPS-stimulated whole blood in healthy men compared with OO, but the effect was not modified by the LA intake.

^* To whom correspondence should be addressed. E-mail: ll{at}life.ku.dk.

Manuscript received 20 November 2008. Initial review completed 5 January 2009. Revision accepted 1 May 2009.

Published online 3 June 2009.