QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, J. Articles by Wu, G. Search for Related Content PubMed PubMed Citation Articles by Wang, J. Articles by Wu, G.

---

Genomics, Proteomics, and Metabolomics

Intrauterine Growth Restriction Affects the Proteomes of the Small Intestine, Liver, and Skeletal Muscle in Newborn Pigs^1,2

³ State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China 100094; ⁴ Department of Animal Science, Texas A&M University, College Station, TX 77843; ⁵ College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, China 410128; ⁶ Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China 410128; ⁷ Xiang-Ya Medical School, Central South University, Changsha, Hunan, China 410078; and ⁸ Protein Chemistry Laboratory, Texas A&M University, College Station, TX 77843

^* To whom correspondence should be addressed. E-mail: g-wu{at}tamu.edu, defali{at}public2.bta.net.cn or yinyulong{at}isa.ac.cn.

Efficiency of nutrient utilization is high in neonates with normal birth weights but is reduced in those with intrauterine growth restriction (IUGR). However, the underlying mechanisms are largely unknown. This study was conducted with the piglet model and proteomics technology to test the hypothesis that IUGR affects expression of key proteins that regulate growth and development of the small intestine, liver, and muscle, the major organs involved in the digestion, absorption, and metabolism of dietary nutrients. Jejunum, liver, and gastrocnemius muscle were obtained from IUGR and normal birth-weight piglets at birth for analysis of proteomes using the 2-dimensional-PAGE MS technology. The results indicate that IUGR decreased the levels of proteins that regulate immune function (immunoglobulins and annexin A1), oxidative defense (peroxiredoxin 1, transferrin, and -crystallin), intermediary metabolism (creatine kinase, alcohol dehydrogenase, L-lactate dehydrogenase, prostaglandin F synthase, apolipoprotein AI, catecho O-methyltransferase, and phosphoglycerate kinase 1), protein synthesis (eukaryotic translation initiation factor-3), and tissue growth (β-actin, desmin, and keratin 10) in a tissue-specific manner. In addition, IUGR increased the levels of proteins that are involved in proteolysis (proteasome -5 and -1 subunits), response to oxidative stress (scavenger-receptor protein and -1 acid glycoprotein), and ATP hydrolysis (F1-ATPase). These novel findings suggest that cellular signaling defects, redox imbalance, reduced protein synthesis, and enhanced proteolysis may be the major mechanisms responsible for abnormal absorption and metabolism of nutrients, as well as reduced growth and impaired development of the small intestine, liver, and muscle in IUGR neonates.

This article has been cited by other articles:

				J. Wang, L. Chen, P. Li, X. Li, H. Zhou, F. Wang, D. Li, Y. Yin, and G. Wu Gene Expression Is Altered in Piglet Small Intestine by Weaning and Dietary Glutamine Supplementation J. Nutr., June 1, 2008; 138(6): 1025 - 1032. [Abstract] [Full Text] [PDF]