QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Online Supporting Material Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rodríguez-Serrano, F. Articles by Aránega, A. Search for Related Content PubMed PubMed Citation Articles by Rodríguez-Serrano, F. Articles by Aránega, A.

---

Biochemical, Molecular, and Genetic Mechanisms

Exogenous Nucleosides Modulate Proliferation of Rat Intestinal Epithelial IEC-6 Cells^1,2

³ Department of Health Science, University of Jaén, 23071 Jaén, Spain; ⁴ Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain; ⁵ Department of Cell Biology, University of Granada, 18071 Granada, Spain; ⁶ Department of Health Sciences, University of Almería, 04120 Almería, Spain; and ⁷ Biopathology and Regenerative Medicine Institute, 18100 Granada, Spain

^* To whom correspondence should be addressed. E-mail: aranega{at}ugr.es.

Exogenous nucleotides are considered semiessential nutritional components that play an important role in intestinal development, maintenance, and recovery from tissue damage. Nucleosides (NS) are the best-absorbed chemical form of nucleotides in the intestinal epithelium. The aim of this work was to clarify, at the cellular level, the effects described in vivo. Under conditions of high intracellular availability of NS, we studied the effects of 2 NS mixtures on the NS uptake and intracellular distribution and on the proliferation, morphology, viability, and cell-cycle phase distribution of rat intestinal epithelial cell line 6. Purine and pyrimidine NS showed a similar uptake profile, but the intracellular incorporation of guanosine was greater than that of uridine, without differences in intracellular distribution. Proliferation assays demonstrated that IEC-6 cell proliferation is increased by a mixture containing thymidine but decreased by one containing uridine. In fact, the antiproliferative effect started at 75 µmol/L, which indicated that it may not be correct to consider concentrations of uridine >75 µmol/L as physiological. Interestingly, these effects were not related to increased cell necrosis or apoptosis or to changed cell morphology but rather to a reduced S-phase and increased G0/G1 phase of the cell cycle. In summary, our results suggest that NS molecules are well-absorbed by rat intestinal epithelial cell line 6 cells, whose proliferation can be promoted or inhibited (according to the NS mixtures used) by a mechanism that is not dependent on the toxicity of the mixtures.