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Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions

Triton WR1339, an Inhibitor of Lipoprotein Lipase, Decreases Vitamin E Concentration in Some Tissues of Rats by Inhibiting Its Transport to Liver¹

² Department of Nutritional Sciences, Nagoya University of Arts and Sciences, Nissin 470-0196, Japan; ³ Department of Food and Nutrition, Sugiyama Jogakuen University, Nagoya 464-8662, Japan

^* To whom correspondence should be addressed. E-mail: saiko{at}nuas.ac.jp.

The aim of this experiment was to clarify the contribution of the -tocopherol transfer activity of lipoprotein lipase (LPL) to vitamin E transport to tissues in vivo. We studied the effect of Triton WR1339, which prevents the catabolism of triacylglycerol-rich lipoproteins by LPL on vitamin E distribution in rats. Vitamin E-deficient rats fed a vitamin E-free diet for 4 wk were injected with Triton WR1339 and administered by oral gavage an emulsion containing 10 mg of -tocopherol, 10 mg of -tocopherol, or 29.5 mg of a tocotrienol mixture with 200 mg of sodium taurocholate, 200 mg of triolein, and 50 mg of albumin. -Tocopherol was detected in the serum and other tissues of the vitamin E-deficient rats, but -tocopherol, - and -tocotrienol were not detected. Triton WR1339 injection elevated (P < 0.05) the serum -tocopherol concentration and inhibited (P < 0.05) the elevation of -tocopherol concentration in the liver, adrenal gland, and spleen due to the oral administration of -tocopherol. Neither -tocopherol administration nor Triton WR1339 injection affected (P 0.05) the -tocopherol concentration in the perirenal adipose tissue, epididymal fat, and soleus muscle despite a high expression of LPL in the adipose tissue and muscle. These data show that -tocopherol transfer activity of LPL in adipose tissue and muscle is not important for -tocopherol transport to the tissue after -tocopherol intake or that the amount transferred is small relative to the tissue concentration. Furthermore, Triton WR1339 injection tended to elevate the serum -tocopherol (P = 0.071) and -tocotrienol (P = 0.053) concentrations and lowered them (P < 0.05) in the liver and adrenal gland of rats administered -tocopherol or -tocotrienol. These data suggest that lipolysis of triacylglycerol-rich chylomicron by LPL is necessary for postprandial vitamin E transport to the liver and subsequent transport to the other tissues.