QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nälsén, C. Articles by Basu, S. Search for Related Content PubMed PubMed Citation Articles by Nälsén, C. Articles by Basu, S.

---

Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions

Dietary (n-3) Fatty Acids Reduce Plasma F₂-Isoprostanes but Not Prostaglandin F₂ in Healthy Humans¹

Cecilia Nälsén^*,2, Bengt Vessby^*, Lars Berglund, Matti Uusitupa^**, Kjeld Hermansen, Gabrielle Riccardi, Angela Rivellese, Len Storlien, Arja Erkkilä^**, Seppo Ylä-Herttuala^**, Linda Tapsell and Samar Basu^*

The KANWU Study Group at ^* Clinical Nutrition and Metabolism, Department of Public Health and Caring Sciences and Uppsala Clinical Research Centre, Uppsala University, Uppsala, Sweden; ^** Department of Clinical Nutrition, University of Kuopio, Kuopio, Finland; Department of Clinical Endocrinology and Metabolism C, Aarhus Sygehus THG, Aarhus University Hospital, Aarhus, Denmark; Department of Clinical and Experimental Medicine, School of Medicine, Federico II University, Naples, Italy; and Department of Biomedical Sciences and Medical Research Unit, University of Wollongong, Wollongong, Australia

² To whom correspondence should be addressed. E-mail: cecilia.nalsen{at}pubcare.uu.se.

(n-3) Fatty acids are unsaturated and are therefore easily subject to oxidization; however, they have several beneficial health effects, which include protection against cardiovascular diseases. The aim of this study was to investigate whether (n-3) fatty acids, with a controlled fat quality in the background diet, affect nonenzymatic and enzymatic lipid peroxidation and antioxidant status in humans. A total of 162 men and women in a multicenter study (The KANWU study) were randomly assigned to a diet containing a high proportion of saturated fatty acids or monounsaturated fatty acids (MUFA) for 3 mo. Within each diet group, there was a second random assignment to supplementation with fish-oil capsules [3.6 g (n-3) fatty acids/d] or placebo. Biomarkers of nonenzymatic and enzymatic lipid peroxidation in vivo were determined by measuring 8-iso-prostaglandin F₂ (8-iso-PGF₂) and prostaglandin F₂ (PGF₂) concentrations in plasma at baseline and after 3 mo. Antioxidant status was determined by measuring plasma antioxidant capacity with an enhanced chemiluminescence assay. The plasma 8-iso-PGF₂ concentration was significantly decreased after 3 mo of supplementation with (n-3) fatty acids (P = 0.015), whereas the PGF₂ concentration was not affected. The antioxidant status was not affected by supplementation of (n-3) fatty acids, but was improved by the background diet with a high proportion of MUFA. We conclude that supplementation with (n-3) fatty acids decreases nonenzymatic free radical–catalyzed isoprostane formation, but does not affect cyclooxygenase-mediated prostaglandin formation.

KEY WORDS: • isoprostanes • prostaglandins • fatty acids • oxidative stress • antioxidant status

This article has been cited by other articles:

				A. E. Barden, K. D. Croft, T. Durand, A. Guy, M. J. Mueller, and T. A. Mori Flaxseed Oil Supplementation Increases Plasma F1-Phytoprostanes in Healthy Men J. Nutr., October 1, 2009; 139(10): 1890 - 1895. [Abstract] [Full Text] [PDF]

				L. Gao, J. Wang, K. R. Sekhar, H. Yin, N. F. Yared, S. N. Schneider, S. Sasi, T. P. Dalton, M. E. Anderson, J. Y. Chan, et al. Novel n-3 Fatty Acid Oxidation Products Activate Nrf2 by Destabilizing the Association between Keap1 and Cullin3 J. Biol. Chem., January 26, 2007; 282(4): 2529 - 2537. [Abstract] [Full Text] [PDF]