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Supplement: Significance of Garlic and Its Constituents in Cancer and Cardiovascular Disease

Aged Garlic Extract Protects against Methotrexate-Induced Apoptotic Cell Injury of IEC-6 Cells^1,2

Toshiharu Horie^*,3, Tiesong Li^*, Kousei Ito^*, Shin-ichiro Sumi and Toru Fuwa

^* Graduate School of Pharmaceutical Sciences, Chiba University, Japan and Central Research Laboratories, Wakunaga Pharmaceutical Company, Japan

³ To whom correspondence should be addressed: E-mail: horieto{at}p.chiba-u.ac.jp.

Gastrointestinal toxicity is one of the most serious side effects of methotrexate (MTX) treatment. The side effects often disrupt the cancer chemotherapy. We previously reported that aged garlic extract (AGE) protects the small intestine of rats from MTX-induced damage. In this study, the protection of AGE against MTX-induced damage of IEC-6 cells originating from the rat jejunum crypt was investigated. MTX decreased the viability of IEC-6 cells, but this effect was prevented by AGE (0.5%). The MTX-induced apoptosis of IEC-6 cells was depressed by AGE. These results indicated that AGE protects IEC-6 cells from the MTX-induced damage. AGE may be useful in cancer chemotherapy with MTX because it reduces MTX-induced intestinal damage.

KEY WORDS: • aged garlic extract • methotrexate • IEC-6 cells • intestinal damage