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Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions

Differential Biliary Excretion of Genistein Metabolites Following Intraduodenal and Intravenous Infusion of Genistin in Female Rats¹

² Department of Pharmacology and Toxicology, ³ Comprehensive Cancer Center Mass Spectrometry Shared Facility, ⁴ Center for Nutrient-Gene Interaction in Cancer Prevention, and ⁵ Purdue University-University of Alabama at Birmingham Botanicals Center for Age-Related Disease, Birmingham, AL 35294

^* To whom correspondence should be addressed. E-mail: jeevan.prasain{at}ccc.uab.edu.

The purpose of this study was to determine whether bioflavonoid glucoside O-conjugates are absorbed from the intestine in the intact form or as their aglycones following hydrolysis by intestinal ß-glucosidases. In this study, the intestinal absorption of genistin, the ß-glucoside of the isoflavone genistein, was examined in anesthetized, adult female rats fitted with indwelling biliary cannulas. To first establish whether genistein, once absorbed, was converted into unique metabolites, genistin was infused into the femoral or portal veins and bile samples quantitatively collected. Analysis of bile samples by HPLC-mass spectrometry revealed that almost full recovery of the genistein component occurred in the form of unreacted genistin (20%) and genistein 7ß-O-glucuronide (80%). However, when genistin was infused into the upper small intestine, only genistein 7ß-O-glucuronide and the aglycone genistein appeared in the bile. There was no evidence for any biliary secretion of the unreacted genistin, thereby excluding its uptake in the intact form from the small intestine in this animal model.