QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Online Supporting Material Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dihal, A. A. Articles by Stierum, R. H. Search for Related Content PubMed PubMed Citation Articles by Dihal, A. A. Articles by Stierum, R. H. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Colorectal Cancer Hazardous Substances DB QUERCETIN

---

Nutrition and Disease

Quercetin, but Not Its Glycosidated Conjugate Rutin, Inhibits Azoxymethane-Induced Colorectal Carcinogenesis in F344 Rats^1,2

³ TNO Quality of Life, Business Unit Biosciences, ⁴ TNO Quality of Life, Business Unit Quality and Safety, Zeist, The Netherlands; ⁵ RIKILT- Institute of Food Safety, Wageningen, The Netherlands; and ⁶ Wageningen University and Research Centre, Division of Toxicology, Wageningen, The Netherlands

^* To whom correspondence should be addressed. E-mail: ashwin.dihal{at}tno.nl.

The effect of the flavonoid quercetin and its conjugate rutin was investigated on (biomarkers of) colorectal cancer (CRC). Male F344 rats (n = 42/group) were fed 0, 0.1, 1, or 10 g quercetin/kg diet or 40 g rutin/kg diet. Two wk after initial administration of experimental diets, rats were given 2 weekly subcutaneous injections with 15 mg/kg body wt azoxymethane (AOM). At wk 38 post-AOM, quercetin dose dependently (P < 0.05) decreased the tumor incidence, multiplicity, and size, whereas tumor incidences were comparable in control (50%) and rutin (45%) groups. The number of aberrant crypt foci (ACF) in unsectioned colons at wk 8 did not correlate with the tumor incidence at wk 38. Moreover, at wk 8 post-AOM, the number and multiplicity of ACF with or without accumulation of ß-catenin were not affected by the 10 g quercetin/kg diet. In contrast, another class of CRC-biomarkers, ß-catenin accumulated crypts, contained less ß-catenin than in controls (P < 0.05). After enzymatic deconjugation, the plasma concentration of 3'-O-methyl-quercetin and quercetin at wk 8 was inversely correlated with the tumor incidence at wk 38 (r = –0.95, P 0.05). Rats supplemented with 40 g rutin/kg diet had only 30% of the (3'-O-methyl-) quercetin concentration of 10 g quercetin/kg diet-fed rats (P < 0.001). In conclusion, quercetin, but not rutin, at a high dose reduced colorectal carcinogenesis in AOM-treated rats, which was not reflected by changes in ACF-parameters. The lack of protection by rutin is probably due to its low bioavailability.

This article has been cited by other articles:

				C. A. Warren, K. J. Paulhill, L. A. Davidson, J. R. Lupton, S. S. Taddeo, M. Y. Hong, R. J. Carroll, R. S. Chapkin, and N. D. Turner Quercetin May Suppress Rat Aberrant Crypt Foci Formation by Suppressing Inflammatory Mediators That Influence Proliferation and Apoptosis J. Nutr., January 1, 2009; 139(1): 101 - 105. [Abstract] [Full Text] [PDF]

				I. Winkelmann, D. Diehl, D. Oesterle, H. Daniel, and U. Wenzel The suppression of aberrant crypt multiplicity in colonic tissue of 1,2-dimethylhydrazine-treated C57BL/6J mice by dietary flavone is associated with an increased expression of Krebs cycle enzymes Carcinogenesis, July 1, 2007; 28(7): 1446 - 1454. [Abstract] [Full Text] [PDF]