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Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions

Dietary Genistein Stimulates Anion Secretion Across Female Murine Intestine¹

² Department of Physiology and ³ Department of Biomedical Sciences, Midwestern University, Glendale, AZ 85308; ⁴ Department of Biomedical Sciences of the College of Veterinary Medicine, ⁵ Dalton Cardiovascular Research Center, ⁶ Veterinary Medical Diagnostic Laboratory of the College of Veterinary Medicine, ⁷ Department of Medical Pharmacology and Physiology of the College of Medicine, and ⁸ Center for Gender Physiology and Environmental Adaptation, University of Missouri, Columbia, MO

^* To whom correspondence should be addressed. E-mail: lalnak{at}midwestern.edu.

Genistein, a naturally occurring isoflavone, augments in vitro epithelial anion transport via activation of the cystic fibrosis transmembrane conductance regulator chloride channel. In this study, we examined whether chronic dietary exposure to 600 mg/kg genistein (600 G) for 1 mo would stimulate anion secretion across wild-type (Wt, normal) murine intestine. Anion secretion was assessed in freshly excised segments of murine jejuna by measuring short circuit current (I_sc) and comparing with jejunal segments from mice fed 0 mg/kg genistein (0 G). Basal and forskolin-stimulated anion secretions were augmented (P < 0.05) in female but not in male mice fed 600 G, compared with their counterparts fed 0 G. Serum genistein concentrations were greater in both female and male mice fed 600 G (3.5–6.9 µmol/L) than those fed 0 G (100 nmol/L). Anion substitution experiments and bumetanide-sensitivity demonstrated that chloride was the major anion mediating the increased secretion. A smaller bicarbonate component was not augmented by consumption of the genistein diet. These data indicate that chronic exposure to dietary genistein stimulates a sex-dependent increase in basal and forskolin-stimulated chloride secretion across murine intestine.