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© 2005 The American Society for Nutritional Sciences J. Nutr. 135:2151-2158, September 2005

Nutrient-Gene Interactions

Mitochondrial Cysteine Desulfurase Iron-Sulfur Cluster S and Aconitase Are Post-transcriptionally Regulated by Dietary Iron in Skeletal Muscle of Rats1

Yih-Fong Liew and Ning-Sing Shaw2

Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan

2To whom correspondence should be addressed. E-mail: nsshaw{at}ntu.edu.tw.

Cysteine desulfurase IscS is required for cellular iron-sulfur protein maturation in eukaryotes and prokaryotes. In this study, we examined the effect of dietary iron intake on the expression in rat skeletal muscle of IscS in relation to 2 iron-sulfur proteins, cytosolic aconitase (c-aconitase) and mitochondrial aconitase (m-aconitase). Three groups of male weanling Wistar rats were used; 1 group was fed an iron-deficient diet (D), and the other 2 groups were pair-fed (P) or freely fed (C) a control (35 mg Fe/kg diet) diet for 1 or 2 wk. At the end of wk 1 and 2, the mitochondrial IscS protein levels in the skeletal muscle of iron-deficient rats had decreased to 45 and 50% of those of the control and pair-fed rats, respectively, whereas the IscS mRNA levels did not differ among the 3 groups, indicating that iron deficiency affected the expression of IscS protein at the post-transcriptional level. Iron deficiency caused a 55–76% reduction in c-aconitase activity and an ~50% reduction in the c-aconitase protein level. The m-aconitase activity and protein level in iron-deficient rats also declined to 50 and 58–64% of the control levels, respectively. Our results indicate that dietary iron modulates mitochondrial IscS protein and aconitase at the post-transcriptional level, and mitochondrial IscS may be associated with this regulation of aconitase in skeletal muscle.

KEY WORDS: • aconitase • cysteine desulfurase • iron deficiency • rats • skeletal muscle






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