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Nutritional Methodology

Identification of Dietary and Endogenous Ileal Protein Losses in Pigs by Immunoblotting and Mass Spectrometry^1,2

Maud Le Gall^*,, Laurence Quillien^**,3, Jacques Guéguen^*, Hélène Rogniaux^* and Bernard Sève

^* INRA, Unité de Recherche sur les Protéines Végétales et leurs Interactions (URPVI), 44072 Nantes Cedex 03; INRA- E.N.S.A.R., Unité Mixte de Recherche sur le Veau et le Porc, 35590 Saint-Gilles; and ^** INRA, Unité de Recherches en Génétique et Ecophysiologie des Légumineuses, Domaine d’Epoisses, 21110 Bretenieres

³To whom correspondence should be addressed. E-mail: quillien{at}epoisses.inra.fr.

Ileal flows and the endogenous or dietary origin of soluble proteins present in ileal digesta were determined in pigs fed diets containing different pea cultivars (Solara, Madria and Eiffel) and micro-ground peas (c.v. Solara). Ileal digesta proteins were analyzed by electrophoresis and densitometry analysis and were identified by LC-MS-MS spectrometry and immunoblotting. The ileal flows of proteins differed (P < 0.1) among the 3 pea cultivars; the flow in pigs fed the Madria-containing diet was higher than that of pigs fed the Eiffel- and Solara-containing diets. The flow was reduced by micro-grinding the peas. The true digestibility of pea proteins and the endogenous losses were not correlated. However, at this intestinal level, protein losses were essentially of endogenous origin (enzymes, antibodies), and from the partly digested pea albumin fraction. Pea lectin and albumin PA1b were totally resistant to gastric and small intestinal digestion and a minor resistant peptide from pea albumin PA2 was detectable. In contrast, the storage proteins, legumin and vicilin, were not detectable by antibodies or by LC-MS-MS.

KEY WORDS: • endogenous protein • pea protein • pigs • digestion • grinding

This article has been cited by other articles:

				M. Le Gall, L. Quillien, B. Seve, J. Gueguen, and J. P. Lalles Weaned piglets display low gastrointestinal digestion of pea (Pisum sativum L.) lectin and pea albumin 2 J Anim Sci, November 1, 2007; 85(11): 2972 - 2981. [Abstract] [Full Text] [PDF]