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Nutritional Immunology

Dietary (n-3) Polyunsaturated Fatty Acids Do Not Affect the In Vivo Development and Function of Listeria-Specific CD4⁺ and CD8⁺ Effector and Memory/Effector T Cells in Mice^1,2

Robert Irons^*,3, Phileno Pinge-Filho^** and Kevin L. Fritsche^*,,4

^* Department of Nutritional Sciences and Department of Microbiology and Molecular Immunology, Division of Animal Sciences, University of Missouri, Columbia, MO 65211; and ^** Department of Pathological Sciences, State University of Londrina, Brazil

⁴To whom correspondence should be addressed. E-mail: FritscheK{at}missouri.edu.

We previously reported that in a mouse model, a diet high in (n-3) PUFA diminishes host survival following an infection from Listeria monocytogenes, a gram-positive bacterial pathogen. In this study we investigated the impact of (n-3) PUFA on the adaptive immune response to L. monocytogenes. BALB/c mice were fed experimental diets either devoid of or rich in (n-3) PUFA from fish oil for 4 wk and then infected with 10⁶ actA-deficient L. monocytogenes. At 7 and 35 d postchallenge, effector and memory/effector T cells in the spleen were enumerated by flow cytometry. Surprisingly, the number of Listeria-specific CD4⁺ and CD8⁺ effector and memory/effector T cells in the spleen was not affected by (n-3) PUFA. Also, the effector cells derived from mice fed either diet were equally capable of conferring protective immunity upon adoptive transfer to naive recipients. Despite our previous data, which demonstrated that (n-3) PUFA profoundly impaired host resistance to L. monocytogenes, pathogen-specific T cell responses were not substantially affected by dietary (n-3) PUFA.

KEY WORDS: • (n-3) PUFA • fatty acid • infection • Listeria • T lymphocytes • mice