QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beagle, B. Articles by Caudill, M. A. Search for Related Content PubMed PubMed Citation Articles by Beagle, B. Articles by Caudill, M. A.

---

Nutrient-Gene Interactions

The Glycine N-Methyltransferase (GNMT) 1289 CT Variant Influences Plasma Total Homocysteine Concentrations in Young Women after Restricting Folate Intake¹

Brandon Beagle, Tai Li Yang, Jean Hung, Edward A. Cogger^*, David J. Moriarty and Marie A. Caudill²

Human Nutrition and Food Science Department, ^* Animal and Veterinary Science Department, and Biological Sciences Department, Cal Poly Pomona University, Pomona, CA 91768

²To whom correspondence should be addressed. E-mail: macaudill{at}csupomona.edu.

ABSTRACT

Glycine N-methyltransferase (GNMT) is a key regulatory protein in folate metabolism, methionine availability, and transmethylation reactions. Perturbations in GNMT may lead to aberrations in homocysteine metabolism, a marker of numerous pathologies. The primary objective of this study was to examine the influence of the GNMT 1289 CT alone, and in combination with the methylenetetrahydrofolate reductase (MTHFR) 677 CT variant, on plasma total homocysteine concentrations in healthy young women (n = 114). Plasma total homocysteine was measured at baseline (wk 0) and after 2 wk of controlled folate restriction (135 µg/d as dietary folate equivalents). Plasma homocysteine concentrations did not differ among the GNMT C1289T genotypes at baseline. However, after folate restriction, women with the GNMT 1289 TT genotype (n = 16) had higher (P = 0.019) homocysteine concentrations than women with the CT (n = 51) or CC (n = 47) genotype. The influence of the GNMT 1289 CT variant on homocysteine was dependent on the MTHFR C677T genotype. In subjects with the MTHFR 677 CC genotype, homocysteine was greater (P 0.05) for GNMT 1289 TT subjects relative to 1289 CT or CC subjects. However, in subjects with the MTHFR 677 TT genotype, plasma homocysteine concentrations did not differ among the GNMT C1289T genotypes. Overall, these data suggest that the GNMT 1289 CT polymorphism influences plasma homocysteine and is responsive to folate intake.

KEY WORDS: • glycine N-methyltransferase • methylenetetrahydrofolate reductase • methionine synthase reductase • homocysteine • folate • women

This article has been cited by other articles:

				Y.-C. Huang, C.-M. Lee, M. Chen, M.-Y. Chung, Y.-H. Chang, W. J.-S. Huang, D. M.-T. Ho, C.-C. Pan, T. T. Wu, S. Yang, et al. Haplotypes, Loss of Heterozygosity, and Expression Levels of Glycine N-Methyltransferase in Prostate Cancer Clin. Cancer Res., March 1, 2007; 13(5): 1412 - 1420. [Abstract] [Full Text] [PDF]