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Nutrient-Gene Interactions

Dietary Rapeseed Oil Affects the Expression of Genes Involved in Hepatic Lipid Metabolism in Atlantic Salmon (Salmo salar L.)^1,2

Ann-Elise O. Jordal³, Bente E. Torstensen, Stephen Tsoi^*,4, Douglas R. Tocher, Santosh P. Lall^* and Susan E. Douglas^*

National Institute of Nutrition and Seafood Research, N-5817 Bergen, Norway; ^* Institute for Marine Biosciences, Halifax, Nova Scotia, Canada B3H 3Z1; and Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK

³To whom correspondence should be addressed. E-mail: ann-elise.jordal{at}nifes.no.

Supplies of marine fish oils (FO) are limited, and sustainable production in aquaculture dictates that alternatives that do not compromise fish health and product quality, such as vegetable oils, must be found. Nutrigenomics will increase our understanding of how nutrition influences metabolic pathways and homeostatic control, and may be used to measure and validate subtle changes in organ-specific, metabolic gene expression signatures. We compared 2 groups of Atlantic salmon fed diets containing 100% FO or 75% rapeseed oil (RO) for 42 wk. A small-scale cDNA microarray was constructed to screen for changes in the expression of lipid metabolism genes in the liver resulting from this partial substitution of RO for FO. 5 fatty acid desaturase gene expression was significantly greater in fish fed 75% RO than in fish fed the control diet; this was confirmed by quantitative real time PCR analysis. In addition, several genes, among these mitochondrial proteins, peroxisome proliferator-activated receptor , as well as other transcription factors, coactivators, and signal transducers, showed significant differential regulation. This partially validated microarray may be used for further gene expression profiling using other dietary comparisons, and for further characterization of selected genes.

KEY WORDS: • fatty acid • microarray • quantitative PCR • lipid metabolism • Atlantic salmon

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