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Nutrition and Cancer

Biotinylation of K12 in Histone H4 Decreases in Response to DNA Double-Strand Breaks in Human JAr Choriocarcinoma Cells^1,2

Nagarama Kothapalli^*, Gautam Sarath and Janos Zempleni^*,**,3

^* Department of Biochemistry, U.S. Department of Agriculture-ARS and Department of Entomology, and ^** Departments of Nutrition and Health Sciences, and Animal Science, University of Nebraska at Lincoln, Lincoln, NE

³To whom correspondence should be addressed. E-mail: jzempleni2{at}unl.edu.

We tested the hypothesis that biotinylation of K12 in histone H4 plays a role in the cellular response to double-strand breaks (DSB) of DNA in human cells. DSB were caused by treating choriocarcinoma JAr cells with etoposide. Biotinylation of K12 in histone H4 decreased by 50% as early as 10–20 min after initiation of treatment with etoposide. Biotinylation returned to initial levels 30–40 min after the addition of etoposide to the medium. Temporal patterns of K12-biotinylation were similar for human lymphoma cells. Phosphorylation of S14 of histone H2B and poly(ADP-ribosylation) of glutamate residues on histone H2A are known markers of DSB in DNA; these modifications increased 10–40 min after alterations in K12-biotinylation occurred. Decreased biotinylation of K12 of histone H4 was specific for DSB but was not detectable in response to single-strand breaks or the formation of thymine dimers. Biotin-deficient choriocarcinoma cells exhibited a 40% decrease in rates of survival in response to etoposide compared with biotin-sufficient controls. These studies suggest that the lack of biotinylation of K12 in histone H4 is an early signaling event in response to DSB.

KEY WORDS: • biotin • DNA damage • double-strand breaks • histone H4

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