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Nutritional Immunology

Gastric Colonization of Candida albicans Differs in Mice Fed Commercial and Purified Diets

Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589 Japan and ^* Research Center, Nippon Beet Sugar Manufacturing Company, Limited, Obihiro 080-0831 Japan

¹To whom correspondence should be addressed. E-mail: ksnym{at}chem.agr.hokudai.ac.jp.

It has been difficult to produce persistent colonization by Candida albicans in the gastrointestinal tract of adult mice without the use of antibiotics and immunosuppressants. We hypothesized that diet influences the colonization of C. albicans and tested the hypothesis. BALB/c mice fed either a commercial rodent diet or a nutritionally adequate mixture of purified ingredients were inoculated i.g. with C. albicans (5 x 10⁷ cells). Gastrointestinal colonization was examined by fecal culture, tissue culture, and histology. Mice fed the purified diet had a high fecal recovery of C. albicans [5–6log₁₀ colony forming units (cfu)/g feces] throughout the experimental period (6 wk), and the major site of colonization was the stomach. C. albicans was undetectable in the feces of mice fed the commercial diet 2 wk after inoculation. Immunosuppressants induced systemic dissemination of C. albicans in mice fed the purified diet. The number of lactobacilli and the concentration of organic acids in the stomach were significantly lower in mice fed the purified diet than in those fed the commercial diet. In vitro culture experiments revealed that acetic and lactic acids suppressed the growth of C. albicans. These results suggest that a reduction in lactobacilli in the stomach of mice fed the purified diet contributed to sustained gastric candidiasis. We therefore propose a novel model of sustained gastric candidiasis by a single i.g. inoculation of C. albicans in healthy adult mice fed a purified diet.

KEY WORDS: • Candidiasis • lactobacilli • purified diet • mice

This article has been cited by other articles:

				N Yamaguchi, R Sugita, A Miki, N Takemura, J Kawabata, J Watanabe, and K Sonoyama Gastrointestinal Candida colonisation promotes sensitisation against food antigens by affecting the mucosal barrier in mice Gut, July 1, 2006; 55(7): 954 - 960. [Abstract] [Full Text] [PDF]