QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chitchumroonchokchai, C. Articles by Failla, M. L. Search for Related Content PubMed PubMed Citation Articles by Chitchumroonchokchai, C. Articles by Failla, M. L.

---

Nutrient Metabolism

Assessment of Lutein Bioavailability from Meals and a Supplement Using Simulated Digestion and Caco-2 Human Intestinal Cells^1,2

Chureeporn Chitchumroonchokchai^*, Steven J. Schwartz^*, and Mark L. Failla^*,**,3

^* Interdisciplinary Ph.D. Program in Nutrition Department of Food Science and Technology and ^** Department of Human Nutrition, The Ohio State University, Columbus, OH

³To whom correspondence should be addressed. E-mail: failla.3{at}osu.edu.

Lutein and zeaxanthin are selectively accumulated in the lens and macular region of the retina. It was suggested that these xanthophylls protect ocular tissues from free-radical damage that can cause cataracts and age-related macular degeneration. Insights regarding the absorption of dietary xanthophylls for delivery to ocular tissues are limited. Our primary objective was to examine factors affecting the transfer of lutein from foods to absorptive intestinal epithelial cells during digestion. Lutein and other carotenoids present in spinach purée and lutein from a commercial supplement were relatively stable during in vitro digestion. Micellarization of lutein and zeaxanthin during the small intestinal phase of digestion exceeded that of ß-carotene and was greater for xanthophylls in oil-based supplements than in spinach. Apical uptake of lutein from micelles by Caco-2 human intestinal cells was linear for at least 8 h, and accumulation from synthetic micelles exceeded that from micelles generated during simulated digestion. Stimulation of chylomicron synthesis resulted in the secretion of 7.6 ± 0.1% of cellular lutein into the triglyceride-rich fraction in the basolateral chamber. These data support the use of simulated digestion and the Caco-2 cell model as effective tools for identifying factors affecting absorption of dietary carotenoids.

KEY WORDS: • lutein • bioavailability • in vitro digestion • micelles • Caco-2 human intestinal cells

This article has been cited by other articles:

				S. Blanquet-Diot, M. Soufi, M. Rambeau, E. Rock, and M. Alric Digestive Stability of Xanthophylls Exceeds That of Carotenes As Studied in a Dynamic in Vitro Gastrointestinal System J. Nutr., May 1, 2009; 139(5): 876 - 883. [Abstract] [Full Text] [PDF]

				M. L. Failla, C. Chitchumroonchokchai, and B. K. Ishida In Vitro Micellarization and Intestinal Cell Uptake of cis Isomers of Lycopene Exceed Those of All-trans Lycopene J. Nutr., March 1, 2008; 138(3): 482 - 486. [Abstract] [Full Text] [PDF]

				O. Kenny, Y. O'Callaghan, and N.M. O'Brien Effects of Ingredient Incorporation into Sausage Meat on the Micellarisation and uptake of {alpha}-tocopherol by Caco-2 Human Intestinal Cells Food Science and Technology International, February 1, 2008; 14(1): 79 - 86. [Abstract] [PDF]

				S. K. Thakkar, B. Maziya-Dixon, A. G. O. Dixon, and M. L. Failla {beta}-Carotene Micellarization during in Vitro Digestion and Uptake by Caco-2 Cells Is Directly Proportional to {beta}-Carotene Content in Different Genotypes of Cassava J. Nutr., October 1, 2007; 137(10): 2229 - 2233. [Abstract] [Full Text] [PDF]

				F. Granado, B. Olmedilla, C. Herrero, B. Perez-Sacristan, I. Blanco, and S. Blazquez Bioavailability of Carotenoids and Tocopherols from Broccoli: In Vivo and In Vitro Assessment Experimental Biology and Medicine, December 1, 2006; 231(11): 1733 - 1738. [Abstract] [Full Text] [PDF]

				C. Chitchumroonchokchai and M. L. Failla Hydrolysis of Zeaxanthin Esters by Carboxyl Ester Lipase during Digestion Facilitates Micellarization and Uptake of the Xanthophyll by Caco-2 Human Intestinal Cells J. Nutr., March 1, 2006; 136(3): 588 - 594. [Abstract] [Full Text] [PDF]

				J. A. Novotny, A. C. Kurilich, S. J. Britz, and B. A. Clevidence Plasma appearance of labeled {beta}-carotene, lutein, and retinol in humans after consumption of isotopically labeled kale J. Lipid Res., September 1, 2005; 46(9): 1896 - 1903. [Abstract] [Full Text] [PDF]

				C. Chitchumroonchokchai, J. A. Bomser, J. E. Glamm, and M. L. Failla Xanthophylls and {alpha}-Tocopherol Decrease UVB-Induced Lipid Peroxidation and Stress Signaling in Human Lens Epithelial Cells J. Nutr., December 1, 2004; 134(12): 3225 - 3232. [Abstract] [Full Text] [PDF]