QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Uritski, R. Articles by Reifen, R. Search for Related Content PubMed PubMed Citation Articles by Uritski, R. Articles by Reifen, R.

---

Nutrient Metabolism

Dietary Iron Affects Inflammatory Status in a Rat Model of Colitis¹

Ram Uritski, Iris Barshack^*, Itzhak Bilkis, Kebreab Ghebremeskel and Ram Reifen²

School of Nutritional Sciences, The Hebrew University of Jerusalem, Rehovot, Israel; ^* Department of Pathology, Sheba Medical Center, Tel Hashomer, Israel; and London Metropolitan University, London, UK

²To whom correspondence should be addressed. E-mail: reifen{at}agri.huji.ac.il.

Iron deficiency anemia is a common feature in inflammatory bowel disease, and oral supplementation is one of the mainstay therapies. However, there is some concern that oral iron supplementation may lead to oxidative stress and exacerbation of inflammation. Our objective was to study the effect of severely deficient, moderately deficient, normal and high iron status on oxidative stress and the course of inflammation in a rat model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The rats were randomly assigned to receive the low-iron diet for 3 (moderately iron-deficient group, n = 16) or 5 (severely iron-deficient group, n = 16) wk, the normal iron diet for 2 wk (normal iron group, n = 16) or the high-iron diet for 2 wk (high-iron group, n = 16). Malondialdehyde concentration, electroparamagnetic resonance measurement, myeloperoxidase activity, and histological analysis were used to evaluate oxidative stress. Noncolitic rats in the high-iron group had higher oxidative stress parameters than those in the other groups. The induction of colitis resulted in severe inflammatory changes in the high-iron and severely iron-deficient groups, and produced higher histological scores in the colon of the normal and high-iron groups. Iron overload, oxidative stress, and inflammation were lower in the moderately iron-deficient group compared with the other 3 groups. In conclusion, we suggest that low rather than normal or high iron supplementation should be considered for the treatment of iron deficiency in inflammatory bowel disease.

KEY WORDS: • iron • inflammatory bowel disease • oxidative stress • rat model

This article has been cited by other articles:

				J. L. Sullivan Stored Iron and Vascular Reactivity Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1532 - 1535. [Full Text] [PDF]