QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nettleton, J. A. Articles by Kurzer, M. S. Search for Related Content PubMed PubMed Citation Articles by Nettleton, J. A. Articles by Kurzer, M. S.

---

Nutrition and Cancer

Plasma Phytoestrogens Are Not Altered by Probiotic Consumption in Postmenopausal Women with and without a History of Breast Cancer¹

Jennifer A. Nettleton, Kristin A. Greany, William Thomas^*, Kerry E. Wangen, Herman Adlercreutz and Mindy S. Kurzer²

Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108; the ^* Division of Biostatistics, University of Minnesota, Minneapolis, MN 55407; and Folkhälsan Research Center and the Division of Clinical Chemistry, Biomedicum, 00014 University of Helsinki, Helsinki, Finland

²To whom correspondence should be addressed. E-mail: mkurzer{at}umn.edu.

Soy phytoestrogens were suggested to reduce the risk of a number of diseases including breast cancer. Given that these compounds are metabolized by bacteria, alteration of intestinal bacteria and enzymes may affect phytoestrogen metabolism. We hypothesized that probiotics, when consumed with soy protein, would increase plasma isoflavones, as well as equol producer frequency, in postmenopausal women. We further hypothesized that these effects would differ between women who have had breast cancer and women who have not. To test these hypotheses, 20 breast cancer survivors and 20 controls completed four 6-wk treatments in a randomized, crossover design: supplementation with soy protein (S) (26.6 ± 4.5 g protein, 44.4 ± 7.5 mg isoflavones/d); soy + probiotics (S+P) (10⁹ colony-forming units Lactobacillus acidophilus DDS+1 and Bifidobacterium longum, 15–30 mg fructooligosaccharide/d); milk protein (M) (26.6 ± 4.5 g protein/d); and milk + probiotics (M+P). Plasma phytoestrogen concentrations did not differ between controls and survivors, although genistein tended to be lower in survivors at baseline (P = 0.15), and during soy (P = 0.16) and milk protein (P = 0.16) consumption. As expected, soy consumption increased plasma phytoestrogen concentrations (P < 0.0001). Plasma phytoestrogen concentrations and the number of equol producers did not differ between the S and S+P diets. At the same time, plasma equol concentrations as well as urinary equol excretion in 2 subjects were more than 7-fold different between the 2 diets. These results indicate that this particular probiotic supplement does not generally affect plasma isoflavones, although the large differences between plasma and urinary equol in some subjects suggest that equol producer status may be modifiable in some individuals.

KEY WORDS: • soy • probiotic • plasma isoflavones

This article has been cited by other articles:

				C. Atkinson, K. M Newton, E. J A. Bowles, M. Yong, and J. W Lampe Demographic, anthropometric, and lifestyle factors and dietary intakes in relation to daidzein-metabolizing phenotypes among premenopausal women in the United States Am. J. Clinical Nutrition, March 1, 2008; 87(3): 679 - 687. [Abstract] [Full Text] [PDF]

				S. Bolca, S. Possemiers, A. Herregat, I. Huybrechts, A. Heyerick, S. De Vriese, M. Verbruggen, H. Depypere, D. De Keukeleire, M. Bracke, et al. Microbial and Dietary Factors Are Associated with the Equol Producer Phenotype in Healthy Postmenopausal Women J. Nutr., October 1, 2007; 137(10): 2242 - 2246. [Abstract] [Full Text] [PDF]

				M. Penza, C. Montani, A. Romani, P. Vignolini, P. Ciana, A. Maggi, B. Pampaloni, L. Caimi, and D. Di Lorenzo Genistein Accumulates in Body Depots and Is Mobilized during Fasting, Reaching Estrogenic Levels in Serum that Counter the Hormonal Actions of Estradiol and Organochlorines Toxicol. Sci., June 1, 2007; 97(2): 299 - 307. [Abstract] [Full Text] [PDF]

				A. A Franke, B. M Halm, L. J Custer, Y. Tatsumura, and S. Hebshi Isoflavones in breastfed infants after mothers consume soy. Am. J. Clinical Nutrition, August 1, 2006; 84(2): 406 - 413. [Abstract] [Full Text] [PDF]

				K. D. R. Setchell and S. J. Cole Method of Defining Equol-Producer Status and Its Frequency among Vegetarians J. Nutr., August 1, 2006; 136(8): 2188 - 2193. [Abstract] [Full Text] [PDF]

				K. Decroos, E. Eeckhaut, S. Possemiers, and W. Verstraete Administration of Equol-Producing Bacteria Alters the Equol Production Status in the Simulator of the Gastrointestinal Microbial Ecosystem (SHIME) J. Nutr., April 1, 2006; 136(4): 946 - 952. [Abstract] [Full Text] [PDF]

				K. D. Setchell, C. Clerici, E. D Lephart, S. J Cole, C. Heenan, D. Castellani, B. E Wolfe, L. Nechemias-Zimmer, N. M Brown, T. D Lund, et al. S-Equol, a potent ligand for estrogen receptor {beta}, is the exclusive enantiomeric form of the soy isoflavone metabolite produced by human intestinal bacterial flora Am. J. Clinical Nutrition, May 1, 2005; 81(5): 1072 - 1079. [Abstract] [Full Text] [PDF]

				J. A. Nettleton, K. A. Greany, W. Thomas, K. E. Wangen, H. Adlercreutz, and M. S. Kurzer The Effect of Soy Consumption on the Urinary 2:16-Hydroxyestrone Ratio in Postmenopausal Women Depends on Equol Production Status but Is Not Influenced by Probiotic Consumption J. Nutr., March 1, 2005; 135(3): 603 - 608. [Abstract] [Full Text] [PDF]

				K. A. Greany, J. A. Nettleton, K. E. Wangen, W. Thomas, and M. S. Kurzer Probiotic Consumption Does Not Enhance the Cholesterol-Lowering Effect of Soy in Postmenopausal Women J. Nutr., December 1, 2004; 134(12): 3277 - 3283. [Abstract] [Full Text] [PDF]