![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Department of Pharmacology & Toxicology, Center for Nutrient-Gene Interaction, University of Alabama at Birmingham, and Purdue University-University of Alabama at Birmingham Botanicals Center for Age-Related Disease, Birmingham, AL 35294
3To whom correspondence should be addressed. E-mail: sbarnes{at}uab.edu.
The weak estrogen-like properties of isoflavonoids were discovered over 50 y ago. In recent years, the overall effects of the isoflavones in soy on human health have been the subject of lively debate largely based on their presumed estrogenic properties. Missing from our knowledge base is the systematic identification of the cellular and biochemical targets of isoflavones and the mechanisms that they influence. Because of the benign effects of isoflavones on cellular integrity, a concentration of the isoflavones can be reached in cell culture models where almost any process can be modulated. Modern systems biology approaches and high dimensional analysis techniques offer new ways to better understand the function of how cells and integrated biological mechanisms respond to compounds such as isoflavones. Data from experiments using DNA microarray analysis for examining the effects of genistein in the developing rat uterus indicate that genistein alters the expression of 6–8 times as many genes as does a physiological estrogen such as 17ß-estradiol. Although these new approaches are exciting, their incipient high dimensionality places considerable strain on the quality of experimental design and meaningful statistical interpretation of the resulting data. In another approach using affinity chromatography methods, DING, a novel genistein-binding protein of yet unknown function, was isolated from human breast cancer MCF-7 cells. Its function remains to be established.
KEY WORDS: • isoflavones • mechanism of action • DNA microarray • high dimensional analysis
This article has been cited by other articles:
|
|
 |
|
  M. Berger, C. A. Rasolohery, R. Cazalis, and J. Dayde Isoflavone Accumulation Kinetics in Soybean Seed Cotyledons and Hypocotyls: Distinct Pathways and Genetic Controls Crop Sci., March 19, 2008; 48(2): 700 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Andres, S. M. Donovan, T. B. Kuhlenschmidt, and M. S. Kuhlenschmidt Isoflavones at Concentrations Present in Soy Infant Formula Inhibit Rotavirus Infection in Vitro J. Nutr., September 1, 2007; 137(9): 2068 - 2073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Chacko, R. T. Chandler, T. L. D'Alessandro, A. Mundhekar, N. K. H. Khoo, N. Botting, S. Barnes, and R. P. Patel Anti-Inflammatory Effects of Isoflavones are Dependent on Flow and Human Endothelial Cell PPAR{gamma} J. Nutr., February 1, 2007; 137(2): 351 - 356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Messina, W. McCaskill-Stevens, and J. W. Lampe Addressing the soy and breast cancer relationship: review, commentary, and workshop proceedings. J Natl Cancer Inst, September 20, 2006; 98(18): 1275 - 1284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. Sacks, A. Lichtenstein, L. Van Horn, W. Harris, P. Kris-Etherton, M. Winston, and for the AHA Nutrition Committee Soy protein, isoflavones, and cardiovascular health: a summary of a statement for professionals from the american heart association nutrition committee. Arterioscler. Thromb. Vasc. Biol., August 1, 2006; 26(8): 1689 - 1692. [Full Text] [PDF]
|
|
|
|
 |
|
 T. A Ryan-Borchers, J. S. Park, B. P Chew, M. K McGuire, L. R Fournier, and K. A Beerman Soy isoflavones modulate immune function in healthy postmenopausal women Am. J. Clinical Nutrition, May 1, 2006; 83(5): 1118 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Trock, L. Hilakivi-Clarke, and R. Clarke Meta-analysis of soy intake and breast cancer risk. J Natl Cancer Inst, April 5, 2006; 98(7): 459 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. Sacks, A. Lichtenstein, L. Van Horn, W. Harris, P. Kris-Etherton, M. Winston, and for the American Heart Association Nutrition Commi Soy Protein, Isoflavones, and Cardiovascular Health: An American Heart Association Science Advisory for Professionals From the Nutrition Committee Circulation, February 21, 2006; 113(7): 1034 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Chacko, R. T. Chandler, A. Mundhekar, N. Khoo, H. M. Pruitt, D. F. Kucik, D. A. Parks, C. G. Kevil, S. Barnes, and R. P. Patel Revealing anti-inflammatory mechanisms of soy isoflavones by flow: modulation of leukocyte-endothelial cell interactions Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H908 - H915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Sacks Dietary Phytoestrogens to Prevent Cardiovascular Disease: Early Promise Unfulfilled Circulation, February 1, 2005; 111(4): 385 - 387. [Full Text] [PDF]
|
|
|
|
|
|
S. Kim, I. Sohn, Y. S. Lee, and Y. S. Lee Hepatic Gene Expression Profiles Are Altered by Genistein Supplementation in Mice with Diet-Induced Obesity J. Nutr., January 1, 2005; 135(1): 33 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Neuhouser Soy and Mammographic Breast Density: Plausible Hypothesis but Limited Evidence in Humans J. Nutr., November 1, 2004; 134(11): 2911 - 2912. [Full Text] [PDF]
|
|
