QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schuler, A. M. Articles by Wood, P. A. Search for Related Content PubMed PubMed Citation Articles by Schuler, A. M. Articles by Wood, P. A.

---

Biochemical and Molecular Actions of Nutrients
Research Communication

Dietary Phytoestrogens Increase Metabolic Resistance (Cold Tolerance) in Long-Chain Acyl-CoA Dehydrogenase–Deficient Mice¹

A. Michele Schuler^*, Stephen Barnes, Barbara A. Gower^** and Philip A. Wood^*,**,2

Departments of ^* Genetics, Pharmacology and Toxicology, and ^** Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL

²To whom correspondence should be addressed. E-mail: paw{at}uab.edu.

We evaluated the role of dietary phytoestrogens (PE) in the disease phenotype of cold intolerance that characterizes long-chain acyl-CoA dehydrogenase–deficient (LCAD–/–) mice, a model of inborn errors of mitochondrial fatty acid ß-oxidation. Male LCAD–/– mice were fed a standard diet containing endogenous PE, a PE-free diet, or a PE-free diet that was supplemented with genistein (250 µg/g diet). The standard diet did not restore complete cold tolerance, but it provided more resistance (P = 0.004) to cold challenge than the PE-free diet. There was a nonsignificant difference (P < 0.07) between LCAD–/– mice fed the genistein-supplemented diet and those fed the PE-free diet. There were no differences in end-point serum glucose concentrations among the 3 groups. Serum FFA were decreased in LCAD–/– mice fed the standard diet compared with those fed the PE-free diet (P = 0.005) and the diet supplemented with genistein (P < 0.001). Serum triglyceride concentrations were greater (P < 0.05) only in LCAD–/– mice fed the genistein-supplemented diet than those fed the standard diet. These results demonstrate the beneficial effects of dietary PE on metabolic tolerance in LCAD–/– mice. Furthermore, they suggest changes that could improve pediatric formula constituents, especially with regard to management of children with inborn errors of fatty acid oxidation.

KEY WORDS: • phytoestrogens • genistein • long-chain acyl-CoA dehydrogenase • cold tolerance